Figure 3.

CSPGs and chondroitin sulfate activate LAR phosphatase in COS-7 cells. A, A dose–response curve indicates the high correlation between color changes (OD at 595 nm) and phosphate gradients. B, C, The bar graphs indicate that stimulation with CSPG (7.5 μg/ml) or CS (400 μg/ml) increases LAR activity (fold changes after CSPGs or CS) in COS-7 cells transfected with LAR-WT, but not with LAR mutants ΔD2 or C1522S. n = 6 wells per group in B and C. *p < 0.05, **p < 0.01, Student's t test. Error bars indicate SEM.