Figure 5. Evolution of Phosphorylation Sites from Acidic Residues Later in Evolution.
(A, B) S. cerevisiae enolase Eno1. Homologs of the S. cerevisiae enolase Eno1 were identified and aligned, and trees were inferred as described for Figure 4. The root of the tree was placed between the archaea and eukaryotes. An expanded view of thirty fungal enolases is shown in panel (B). The fungus Cryptococcus neoformans was used as an outgroup to define the root of the tree.
(C) Amino acid sequences close to the phosphosite for a few selected enolase homologs. See also Table S3.