Fig. 4.

ChIP–qPCR assay representing association of AP-2alpha at hZip1 and hZip3 promoter regions in vivo. Target DU-145 (A) or LNCaP (B) ChIP-DNA increased after cells were treated with 5-aza-CdR. Bars represent fold change of 2^−(ΔΔCt) of target ChIP-DNA in treated cells over 2^−(ΔΔCt) of target ChIP-DNA in untreated cells. Normalized against input Ct values of hZip1 and hZip3 promoters ChIP-DNA were normalized to the GAPDH promoter ChIP-DNA samples recovered with anti-AP-2alpha antibodies. DU-145 and LNCaP cells were preincubated with 5 μM of 5′-aza-CdR for 48 h with an additional 24 h incubation in cell medium alone. After incubation, cells were harvested and ChIP–qPCR was performed. The qPCR reactions were ran in triplicates. The representative data of one of the three ChIP–qPCR experiments are presented. Error bars represent ±SD of triplicates.