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. Author manuscript; available in PMC: 2011 Nov 18.
Published in final edited form as: Neuron. 2003 Jul 17;39(2):227–239. doi: 10.1016/s0896-6273(03)00366-0

Figure 4. The Htt Amino Terminus Facilitates InsP3R1 Activity in the Presence of HAP1A.

Figure 4

(A) Effects of HAP1A, Htt-N-15Q, and Htt-N-138Q on activity of recombinant InsP3R1 in planar lipid bilayers in the presence of 100 nM InsP3. Each current trace corresponds to 10 s (2 s for expanded traces) of current recording from the same experiment.

(B) The average InsP3R1 open probability (Po) in the presence of 100 nM InsP3 is calculated for a 5 s window of time and plotted for the duration of an experiment. The times of InsP3, HAP1A, Htt-N-15Q, and Htt-N-138Q additions to the bilayer are shown above the Po plot. Data from the same experiment are shown on (A) and (B). Similar results were obtained in two independent experiments or in experiments when HAP1A and Htt-N-15Q (n = 4) or HAP1A and Htt-N-138Q (n = 4) were premixed.