. 2011 Oct 31;8:488. doi: 10.1186/1743-422X-8-488

Table 2.

Infection rates of two ACLSV hosts following mechanical inoculation of in vitro transcripts obtained from ACLSV FL-cDNA under the T7 promoter synthesized using two different PCR enzymes

Enzyme used for LD-PCR	Infected/inoculated plants (% infected)^a,b

	Chenopodium quinoa	Nicotiana occidentalis 37B
Advantage GC^c	8/36 (22%)	0/22 (0%)
Phusion^d	30/36 (83%)	0/22 (0%)
water inoculation control^e	0/12 (0%)	0/12 (0%)

a: the results shown are the sum of two inoculation experiments performed with transcripts deriving from PCR products resulting from different amplification reactions.

b: plants were mechanically inoculated using 5 μg of transcripts per plant.

c: Advantage^®GC Genomic LA Polymerase Mix (Clontech)

d: Phusion^®High Fidelity DNA Polymerase (Finnzyme)

e: plants were rub inoculated using the DEPC-treated sterile water used to resuspend the in vitro transcripts.