Table 3.
Infectivity on Chenopodium quinoa plants and number of infectious plasmids in plasmid pools obtained upon cloning by homologous recombination in yeast cells of ACLSV FL-cDNA under the control of the T7 promoter (T7-cDNA) or of the duplicated 35S promoter (70S-cDNA)
| Plasmid pools | Infected/inoculated C. quinoa plantsa | Infectious plasmids per pool |
|---|---|---|
| T7-cDNA Pool 1 | 0/10 | 0 |
| T7-cDNA Pool 2 | 0/26 | 0 |
| T7-cDNA Pool 3 | 10/16 | 1 |
| T7-cDNA Pool 4 | 0/26 | 0 |
| 70S-cDNA Pool 1 | 5/6 | 1 |
| 70S-cDNA Pool 2 | 4/6 | 1 |
| 70S-cDNA Pool 3 | 0/12 | 0 |
a: 3 μg of capped transcripts per C. quinoa plant were used for inoculation of transcripts from T7-cDNA plasmid pools while about 160 ng of plasmids were used per plant for biolistic inoculation of 70S-cDNA pools (2 shots per plant).