Figure 8. Ca²⁺ entry is not altered with age in mouse chromaffin cells.

Cells from each age were loaded with the Ca²⁺-sensitive dye, Fluo-4, and stimulated for 60 sec with 70 mM K⁺ solution. The increase in fluorescence reflects Ca²⁺ entry and we find that the total increase in fluorescence (area under the curve; A) and the peak fluorescence change (ΔF; B) are unchanged at any age. Data is mean ± SEM, significance tested using one-way ANOVA. n = 16, 9 and 8 cells from P0 (white bar), 1 month (striped bar) and 6 month (grey bar), respectively. Cells were obtained from >3 mice in each age group.