Figure 2.

Site-directed mutagenesis of the 43 region (StuI to HincII) identified an Ets binding site on the CD5 promoter as necessary for maximal CD5 promoter. Each of the three potential transcription factor binding sites- CCAAT, κE2, and Ets, was mutated on the StuI B reporter construct as described in Materials and Methods. EL4 cells were transiently transfected with StuI B, mutC StuI B, mutK StuI B, mutE StuI B, or HincII B constructs, and with pON405 LacZ reporter plasmid as internal control. Each bar value represents the average of three independent transfections in one experiment, with standard deviation as error bars.