Skip to main content
. 2001 May 22;2:5. doi: 10.1186/1471-2199-2-5

Figure 6.

Figure 6

EMSA analysis with oligonucleotides corresponding to the CD5X region. The 32P-labeled CD5X oligonucleotide (5' AGG CCT AAG TTG ACA GTT CAA CTT CAA ACA CTC GAG 3') was incubated with EL4 nuclear extract (lane 2-6). Competitive EMSA was performed with 100-fold excess specific oligonucleotide, X oligos (lane 3), or irrelevant oligos: Y oligos (lane 4); CK oligos (lane 5); and KE (lane 6). The protein:DNA complexes specific to the CD5X oligonucleotides are indicated with arrows. F indicates the migration of the free probe. 

EMSA analysis with oligonucleotides corresponding to the CD5Y region. The 32P-labeled CD5Y oligonucleotide (5' AGG CCT CAC AGG CCC ACA CTG CCT GCT TCC CTG GAG 3') was incubated with EL4 nuclear extract and subsequently resolved in EMSA. The protein:DNA complexes specific to the CD5Y oligonucleotides were indicated with arrows. In lane 1 is 32P-labeled CD5Y oligos alone; lanes 2-6, 32P labeled CD5Y oligos plus EL4 nuclear extract. Competitive EMSA are shown on lanes 3-6 with addition of 100-fold molar excess oligonucleotides for competition: CD5Y (lane 3), CD5X (lane 4), CK (lane 5), and KE (lane 6). The protein:DNA complex specific to the CD5Y oligonucleotides are indicated with arrows. F indicates the migration of the free probe.