. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: Exp Mol Pathol. 2011 Jul 23;91(3):673–681. doi: 10.1016/j.yexmp.2011.06.012

TABLE 2.

Monocyte/macrophage adhesion and fusion to form FBGC in vitro.

Morphology	Culture Time	Adherent Cells (Nuclei per mm² )^a	Macrophage Fusion(%)^b
Monocytes	Day 0	2013 ± 214	n.a.^c
Macrophages (short-term)	Day 3	1056 ± 50	n.a.
Macrophages (long-term)	Day 10	1067 ± 40	6 ± 2
Fusing macrophages/FBGC	Day 1O + IL-4	1073 ± 42	71 ± 3

Nuclei per mm² in May-Grünwald/Giemsa-stained cells were counted at 20× magnification on an inverted stage microscope

Percent macrophage fusion represents the mean number of nuclei ± SEM within FBGC (cells with > 2 nuclei) compared to total nuclei counted (n = 3).

not applicable