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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: FEBS J. 2011 Oct 20;278(23):4633–4648. doi: 10.1111/j.1742-4658.2011.08365.x

Figure 3. Relative affinity of PMT binding to phospholipids and lactosylceramide.

Figure 3

Images of TLC plates loaded with incremental amounts of lactosylceramide (LacCer) and developed in a solvent system of 85:15:1 (see experimental section) or with phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM) and developed in a solvent system of 75:25:4. Right panels are images of overlays with the indicated radiolabeled proteins recorded by phosphorimager. Left panels are images of the corresponding LC plates subsequently stained with CuSO4.