Fig. 5.

Characteristics of anticancer peptides: (A) peptide length; (B) net charge; (C) hydrophobic content (% hydrophobic amino acids; http://aps.unmc.edu/AP/design/design_improve.php); (D) hydrophobicity of peptides expressed as transfer free energy of peptides from water to n-octanol (ΔG_woct) taking into account the contribution of both endgroups (COO⁻ and NH₃⁺), in case of C-amidation or N-acetylation ΔG_woct decreases by 3.6 kcal/mol and 2.3 kcal/mol, respectively (http://blanco.biomol.uci.edu/mpex/) (Wimley et al., 1996), n.c., not calculated for peptides >70 amino acids; (E) structural characteristics.