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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Histopathology. 2011 Oct 18;59(5):1018–1023. doi: 10.1111/j.1365-2559.2011.03959.x

Table 1.

Descriptive Summary of Clinical and Pathologic Findings.

Subject Age Repeat
Number		 Menstrual History
at
Time of Surgery		 Reason for
Surgery		 Ovarian Pathology (H&E) Ubiquitin Immunohistochemistry
Established Fragile X Premutation Carriers
Case 1 31 75 Postmenopausal
since age 20 yrs		 Ovarian cysts Follicles and Stroma:
 Atretic follicles; ovarian stroma present and evaluable.
Epithelium:
 Cortical inclusion cysts.
 Mucinous cyst.		 Follicles and Stroma:
Intranuclear inclusions in stromal cells: up to 3 per 10 high power fields.
Non-specific nuclear staining seen in perivascular stromal cells.
Epithelium:
 Non-specific nuclear staining.
Case 2 42 100 28 day cycle Adenomyosis Follicles and Stroma:
 Atretic follicles; ovarian stroma present and evaluable.
Epithelium:
 Cortical inclusion cysts.		 Follicles and Stroma:
Intranuclear inclusions in stromal cells: up to 20 per 10 high power fields.
Epithelium:
 Minimal to no non-specific staining.
Case 3 39 100 Irregular, infertility,
menometrorrhagia
worsening with oral
contraceptives.		 Menometror-
rhagia, pelvic
pain
(Ovarian biopsy
only)		 Follicles and Stroma:
 No follicles identified; ovarian stroma present and
evaluable.
Epithelium:
 Endometriosis.		 Follicles and Stroma:
Intranuclear inclusions in stromal cells: more than 50 per 10 high power
fields. Non-specific nuclear and cytoplasmic staining seen in ovarian
stromal cells.
 Epithelium:
Non-specific nuclear and cytoplasmic staining seen in endometriotic stromal
and epithelial cells.
Case 4 72 79 Postmenopausal Uterine
bleeding, and
biopsy proven
endometrial
adenocarcinoma		 Follicles and Stroma:
 Atretic follicles; fibroma; ovarian stroma present and
evaluable.
Epithelium:
 Cortical inclusion cysts.		 Follicles and Stroma:
Intranuclear inclusions in stromal cells: more than 50 per 10 high power
fields. Non-specific nuclear and cytoplasmic staining seen in ovarian and
perivascular stromal cells.
Epithelium:
 Non-specific nuclear staining.
Case 5 44 62 Irregular menses
every 3 to 4 months,
with 6 months of
amenorrhea,
infertility and FSH
34 IU/L		 Complex right
ovarian cyst and
BRCA1
mutation		 Follicles and Stroma:
 Cystic follicle (functional); ovarian stroma present and
evaluable.
Epithelium:
 Cortical inclusion cysts.		 Follicles and Stroma:
Intranuclear inclusions in stromal cells: up to 7 per 10 high power fields.
Non-specific cytoplasmic staining; granulosa > theca cells. Non-specific
nuclear and cytoplasmic staining seen in ovarian and perivascular stromal
cells.
Epithelium:
 Non-specific nuclear staining.
Control Ovarian Tissue
Control 1 27 Normal
(31, 36)		 Regular 28 days Ovarian cyst Follicles and Stroma:
 No follicles identified. Ovarian stroma present and
evaluable.
Epithelium:
 Mature cystic teratoma (squamous, sebaceous, thyroid,
and glial-type tissue present).		 Follicles and Stroma:
 No intranuclear inclusions in stromal cells. Non-specific nuclear staining
seen in ovarian and perivascular stromal cells.
Epithelium:
 Non-specific nuclear and cytoplasmic staining seen in epithelial and
ectodermal elements of the mature cystic teratoma.
Control 2 47 Normal
(31, 31)		 Unknown Follicles and Stroma:
 Cystic follicle (functional); ovarian stroma present and
evaluable.
Epithelium:
 Cortical inclusion cysts		 Follicles and Stroma:
No intranuclear inclucions in stromal cells. Non-specific cytoplasmic
staining; granulosa > theca cells. Non-specific nuclear and cytoplasmic
staining seen in ovarian and perivascular stromal cells.
Epithelium:
 Minimal non-specific nuclear staining.
Control 3 70 Unknown Postmenopausal Follicles and Stroma:
 Atretic follicles; ovarian stroma present and evaluable.
Epithelium:
 Cortical inclusion cysts.		 Follicles and Stroma:
 No intranuclear inclusions in stromal cells. Non-specific nuclear staining
seen in ovarian and perivascular stromal cells.
Epithelium:
 Minimal non-specific nuclear staining.
Control 4 61 Unknown Postmenopausal Prophylactic
oophorectomy
for strong family
history of breast
cancer		 Follicles and Stroma:
 Atretic follicles; ovarian stroma present and evaluable
Epithelium:
 Ovarian surface and fallopian tube only.		 Follicles and Stroma:
 Intranuclear inclusions in stromal cells: up to 2 per 10 high power fields.
 Minimal non-specific staining seen in ovarian stromal cells.
Epithelium:
 Minimal non-specific nuclear and cytoplasmic staining.

Notes: Field diameter of high power field: 0.63 mm.

The ubiquitin-positive intranuclear inclusions observed measured 3 to 5 micrometers, and could not be seen on H&E sections.