. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: J Med Virol. 2011 Nov 3;84(1):152–161. doi: 10.1002/jmv.22147

Table 3.

Distribution of Synonymous and Non-synonymous Nucleotide Substitutions in Different Subject Categories

Region	dN			dS			dN:dS
Region	Viremia/nephropathy	Transplanted-viruria	Healthy-viruria	Viremia/nephropathy	Transplanted-viruria	Healthy-viruria	Viremia/nephropathy	Transplanted-viruria	Healthy-viruria
Whole VP1	0.0015±0.0012¹ (0.0002–0.0044)	0.0014±0.0004 (0.0006–0.0019)	0.002±0.001 (0.001–0.67)	0.001 ± 0.0007² (0–0.0024)	0.0046±0.0043 (0.0012–0.014)	0.005 ± 0.0032 (0.0023–0.064)	1.5 ± 1.08³ (0.125–3.375) (n=12)	0.4±0.16³ (0.11–0.59) (n=7)	0.45±0.16³ (0.005–0.62) (n=7)
BC Loop	0.008 ± 0.009¹ (0–0.0317)	0.0021±0.0015 (0–0.0036)	0.005±0.009 (0–0.0234)	0.0004±0.0014 (0–0.053)	0.0036±0.0063 (0–0.0139)	0.005±0.009 (0–0.0238)	2.58 (n=1)	0.25±0.014 (0.24–0.26) (n=2)	0.7±0.36 (0.47–0.98) (n=2)
DE Loop	0.0024±0.0032 (0–0.0101)	0.0014±0.0019 (0–0.0046)	0.0038±0.0045 (0–0.0115)	0.0011±0.0029 (0–0.0081)	0.005±0.007 (0–0.0167)	0.0037±0.008 (0–0.018)	0.41±0.19 (0.28–0.54) (n=2)	0.28±0.006 (0.28–0.29) (n=3)	0.43 (n=1)
HI Loop	0.0011 ± 0.0029¹ (0–0.0102)	0.0016 ± 0.002 (0–0.0059)	0.0017 ± 0.003 (0–0.0063)	0.002 ± 0.005 (0–0.0151)	0.013 ± 0.034 (0–0.0167)	0 (0)	0 (0) (n=2)	0 (n=1)	N/A (dS=0 for all sequences)

Data is presented as mean +/− sd with the range indicated in parentheses.

N/A: not applicable

P<0.05, dN, BC Loop vs whole VP1 region and HI Loop in Viremia/nephropathy group.

P<0.05, dS Viremia/nephropathy vs Healthy-viruria in whole VP1 gene.

P<0.05, dN/dS, Viremia/nephropathy vs Transplanted-viruria & Healthy-viruria groups in whole VP1 gene.

Putative BC loop (nucleotides 169 – 267, Dun numbering, Acc. V01108), DE loop (# 382 – 438), and HI loop (# 805 – 835) were identified using the published literature: (40, 41)