Figure 4. DNMT1 and EZH2 Form Nuclear Foci after H₂O₂ Treatment that Co-localize with γ-H2AX.

(A) NCCIT cells were untreated or treated with 2 mM H₂O₂ for 30 minutes. Immunofluorescence analysis was performed using the indicated antibodies. White arrows indicate examples of foci. White scale bar is 5 μm. (B) More than 50 nuclei from cells in (A) were scored per antibody in at least two independent experiments. Graphs represent the sample mean +/− SEM. Grey and black bars are untreated and H₂O₂ treated cells, respectively. (C) More than 50 nuclei from cells in (A) were scored per antibody in at least two independent experiments. Graphs represent the sample mean +/− SEM. Black bars are H₂O₂ treated cells. See also Figure S3.