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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Cancer Cell. 2011 Nov 15;20(5):661–673. doi: 10.1016/j.ccr.2011.10.012

Figure 6. Contribution of IL-6 to lineage determination in vivo.

Figure 6

(A) Experimental scheme. 5×106 wild type (tWT: CD45.2 GFP+) and 5×106 non-induced BA+(CD45.2 GFP-) BM cells were co-transplanted into lethally irradiated recipients (CD45.1). Transplanted mice were maintained on doxycycline for 2 months to achieve stable reconstitution before inducing BA expression by doxycycline withdrawal. Mice were analyzed either pre or post BA-induction, when mice displayed signs of CML disease.

(B) Representative FACS plots showing contribution of tWT and tBA cells to the myeloid (Mac1+/Gr1+) BM output pre (top) and post (bottom) induction (n≥2).

(C) Concentration of IL-6 in the serum of transplanted mice pre and post induction. Results are expressed as mean ± SEM (n=2-4).

(D) Representative FACS plots showing contribution of tWT and tBA cells to the indicated BM compartments post induction (n=2).