Figure 3.

Transcript accumulation of phenylpropanoid genes during seedling development. Arabidopsis seedlings were germinated and grown under aseptic conditions on modified Murashige-Skoog agar plates (Lorenzen et al., 1996) in light (L; 16-h light/8-h dark photoperiod), in darkness (D), or in darkness for 4 d before being shifted to light conditions (D→L). Total RNA was extracted on the days indicated and RNA analysis was performed using probes from cDNAs of the indicated genes. A, Total RNA from wild-type seedlings probed with the F5H cDNA. The lower panel illustrates ethidium-bromide staining of rRNA as a loading control. B, Total RNA from wild-type seedlings probed with cDNAs corresponding to the phenylpropanoid genes indicated. C, Total RNA from a fah1-2: F5H(HX) transgenic line probed with the F5H cDNA. All blots were exposed to film for 24 h except PAL2, which was exposed for 48 h.