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Accumulation of F5H transcript in developing siliques. Siliques were collected in pairs from 5-week-old plants of the lines indicated, beginning with the first expanding silique. Total RNA was extracted and probed with the F5H cDNA in RNA analysis. Blots were exposed to film for 24 h (fah1-2:35S-F5H and fah1-2:C4H-F5H) or 48 h. The bottom panel illustrates ethidium-bromide staining of rRNA as a loading control. Col, Arabidopsis ecotype Columbia.
