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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Amino Acids. 2011 Aug 18;42(2-3):703–710. doi: 10.1007/s00726-011-0986-z

Figure 2. Genotyping of pups and blastocysts (E3.5) from Eif5a+/gt intercross (A and B) and from Dhps+/gt intercross (C and D).

Figure 2

Genomic DNA was isolated from tail snips of every litter mates (pups) from one Eif5a+/gt intercross (A) or one Dhps+/gt intercross (C), from every blastocysts isolated from another Eif5a+/gt intercross (B) and Dhps+/gt intercross (D) and were used as templates for PCR genotyping. For genotyping of pups shown in A and C, both sets of primers (indicated under Materials and Methods) were included in one PCR mixture to detect the targeted allele as well as the wild type allele in one tube. For genotyping of blastocysts (B and D), two separate PCR reactions were carried out with either mutant allele specific primer sets or with wild type specific primer sets.