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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Amino Acids. 2011 Aug 18;42(2-3):703–710. doi: 10.1007/s00726-011-0986-z

Figure 4. Reduced levels of eIF5A-1 and DHPS proteins and reduced BrdU incorporation in Eif5agt/gt and Dhpsgt/gt blastocysts (E3.5) cultured in vitro.

Figure 4

The cells from the 7-day in vitro culture of blastocysts (E3.5) were labeled with BrdU. Cell proliferation was estimated from incorporation of BrdU by using anti-BrdU and anti-mouse IgG-fluorescein. To determine the levels of eIF5A-1 and DHPS in these cells, the cells were treated with rabbit polyclonal primary antibodies, anti-eIF5A and anti-DHPS, respectively, and then stained using anti-rabbit IgG-Cy3. DAPI was used for counterstaining of nuclei.