Table 1.
Early embryonic lethality of homozygous disruption of Eif5a (A) and Dhps (B) genes. Heterozygous Eif5a+/gt male and female or Dhps+/gt male and female were mated. On day 3.5, 6.5, 7.5 and 8.5, embryos were flushed out or detached, genomic DNA was isolated for PCR genotyping. Multiple matings were performed for Eif5a+/gt (10 intercrosses) and Dhps+/gt (11 intercrosses) for genotyping of the pups. Sum of the numbers from multiple matings is shown for each genotype.
| A Eif5A-1+/gt intercross
| ||||
|---|---|---|---|---|
| Stage | Number/litter | Genotype
|
||
| +/+ | +/gt | gt/gt | ||
| Mice (3 weeks) | 65/10 | 23 | 42 | 0 |
| E8.5 | 29/3 | 9 | 20 | 0 |
| E7.5 | 9/1 | 1 | 8 | 0 |
| E6.5 | 5/1 | 0 | 4 | 1 |
| E3.5 | 52/9 | 11 | 19 | 21 |
| B Dhps+/gt intercross
| ||||
|---|---|---|---|---|
| Stage | Number/litter | Genotype
|
||
| +/+ | +/gt | gt/gt | ||
| Mice (3 weeks) | 52/11 | 12 | 40 | 0 |
| E8.5 | 8/1 | 2 | 6 | 0 |
| E7.5 | 9/1 | 3 | 6 | 0 |
| E6.5 | 25/3 | 9 | 16 | 0 |
| E3.5 | 25/4 | 7 | 13 | 5 |