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Representation of interactions of Npt2a/PDZK1 and Npt2c/PDZK1. Npt2a and Npt2c interact with different PDZ domains of PDZK1, PDZ3 and PDZ2, respectively. Therefore, during the interaction of the pairs Npt2a/PDZK1 and Npt2c/PDZK1, the distance of the fluorophores is likely to be different between the 2 pairs and the FRET efficiencies are not comparable. For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.
