Table II.
Efficiency of the aldehyde mixture (3% [w/v] paraformaldehyde and 0.5% [v/v] glutaraldehyde in PBS) to link cytokinin bases (IP, DHZ, and zeatin) and cytokinin ribosides (IPA, DHZR, and ZR) to BSA and plant material
| BSAa | Plant Materialb | |
|---|---|---|
| A405 | % | |
| BSA | 0.2 | – |
| IP | 3.1 | 23 |
| IPA | 0.5 | 6 |
| DHZ | 3.0 | 11 |
| DHZR | 0.2 | 2 |
| Zeatin | 3.0 | 11 |
| ZR | 0.2 | 2 |
a
A405 of the reaction product of the alkaline phosphatase. To reveal binding of cytokinin ribosides or bases to BSA, immunostaining with alkaline phosphatase linked to the secondary antibody was performed.
b
Percentage of radioactive tracer linked to 1 g of plant material. Coupling of radioactive cytokinin bases and ribosides to plant tissues was estimated by liquid scintillation, and the percentages were calculated toward the total amount of radioactivity used in the assay.