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Ethanol has no effect on caveolin-1 association with lipid rafts. A) CGN are treated as described, either no addition (Control) or 25 mM ethanol for 1 h (EtOH), prior to preparation of lipid rafts. Lipid rafts (LR) were isolated from non-lipid rafts (N) by sucrose density gradient, pooled, protein precipitated and immunoblotted for caveolin-1. Blots were stripped and reblotted for L1 and CTxB. B) Blots (n=3) were quantified by densitometry. Mean values +/− SD are shown.
