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. Author manuscript; available in PMC: 2012 Nov 1.

Published in final edited form as: J Neurochem. 2011 Oct 11;119(4):859–867. doi: 10.1111/j.1471-4159.2011.07467.x

L1 mediated neurite outgrowth is dependent on lipid rafts. A) Immunoblot of L1 in sucrose density gradient fractions of CGN treated with or without MBCD. All detectable L1 colocalizes with the transferrin receptor (TfR), a marker for the non-lipid raft fraction. B) Neurite length of CGN in the presence of various reagents. Cells are plated on either PLL alone (Control) or PLL and L1-Fc (L1-Fc). Ethanol (25 mM) and/or MBCD (4mM) are added 2 h after plating. Cells are fixed 12 h after plating, and neurite length is measured. Shown is mean neurite length +/− SD. Paired t test: a, p<0.0002 from control; b, p< 0.0002 from L1 -Fc; c, p<0.02 from Control; d, p not significant from Control; e, p not significant from L1-Fc+MBCD nor Control.

L1 mediated neurite outgrowth is dependent on lipid rafts. A) Immunoblot of L1 in sucrose density gradient fractions of CGN treated with or without MBCD. All detectable L1 colocalizes with the transferrin receptor (TfR), a marker for the non-lipid raft fraction. B) Neurite length of CGN in the presence of various reagents. Cells are plated on either PLL alone (Control) or PLL and L1-Fc (L1-Fc). Ethanol (25 mM) and/or MBCD (4mM) are added 2 h after plating. Cells are fixed 12 h after plating, and neurite length is measured. Shown is mean neurite length +/− SD. Paired t test: a, p<0.0002 from control; b, p< 0.0002 from L1 -Fc; c, p<0.02 from Control; d, p not significant from Control; e, p not significant from L1-Fc+MBCD nor Control.