Fig. 2.

The effects of the methionine- and choline-deficient diet (MCD) diet and dietary oleate on hepatic carbohydrate responsive element-binding protein (ChREBP) and apolipoprotein B (apo B) expression. Immunohistochemical detection of ChREBP in liver paraffin-embedded sections. Scale bars, 50 µm. (A) Chow diet-fed mice. (B) MCD diet-fed mice. (C) MCD diet with oleate (0.5 mg/g/day for 4 weeks)-fed mice. (D) Real-time reverse transcription-polymerase chain reaction (RT-PCR) quantification of ChREBP mRNA in mouse liver samples from each group. (E) Real-time RT-PCR quantification of APOB100 mRNA in mouse liver samples from each group. AU, arbitrary unit; Chow, normal chow diet; OMCD, MCD diet with oleate. ^aP<0.05 vs. chow, ^bP<vs. MCD.