Figure 6.
Tyrosine phosphorylation levels of KIR2.3 channels. (A) Upper panel, protein lysates were immunoprecipitated with anti-KIR2.3 antibody, and then Western blots were prepared and probed with the anti-phosphotyrosine (Tyr(P)) antibody. Lower panel, Western blots of cell lysates, probed with the anti-KIR2.3 channel antibody. (B) Histogram summarizes the mean values of the relative levels of tyrosine-phosphorylated KIR2.3 channel protein analysed by densitometry. The amount of protein from the immunoprecipitation (as in A) was normalized to those from the Western blots. Phosphorylated protein levels (n = 5 experiments) are expressed as a percentage of the vehicle control. **P < 0.01, *P < 0.05 vs. vehicle control, #P < 0.05 vs. genistein or AG556 alone.