Table 1.

Quantitative RT-PCR characterization of the myometrial endocannabinoid system

	CB1	CB2	TRPV1	FAAH	NAPE-PLD	β-Actin
Cell type	Ct	Ct	Ct	Ct	Ct	Ct
ULTR	11.2 ± 0.43	16.7 ± 0.13	9.2 ± 0.40	15.4 ± 0.94	9.5 ± 0.24	16.8 ± 0.15
Primary	16.1 ± 0.49	17.5 ± 0.21	11.2 ± 0.26	17.1 ± 0.51	10.1 ± 0.05	17.6 ± 0.35

Mean cycle threshold (Ct) values for CB₁ and CB₂ receptors, TRPV1 channels, FAAH, NAPE-PLD and β-actin in ULTR and primary myometrial cells. Samples were assayed in triplicate from three separate extractions from n = 3 separate patients and expressed as mean Ct values ± SEM. cDNA from ULTR and primary myometrial cell lysates were amplified with specific, commercially verified, primer/probe sets. All data are related to ΔRn; the change in the normalized reporter fluorescence, relative to basal.