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Immunoblot analysis of Rubisco accumulation in transgenic leaves. Total cellular protein (1 μg per lane) was from Nt-pIK83-1 (lane S, rbcL-S), Nt-pIK83-4 (lane R, rbcL-R), and Nt-pIK84-15 (lane C, rbcL-C) plants. For comparison, a dilution series (1:10, 1:5, 1:3, and 1:2) of a wild-type tobacco extract (T) is also shown. The blots were probed with antibodies to the spinach Rubisco large (L) and small (S) subunits (Kanevski and Maliga, 1994).
