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. 2011 Apr 18;4(6):938–946. doi: 10.1093/mp/ssr030

Figure 6.

Figure 6

Drought-Tolerance Assays of Control and pub19 mutant Plants and Effects of ABA on Stomatal Aperture of WT and pub19-1 Mutant Plants.

(A) Two-week-old plants were used for the drought-tolerance assays. Plants were grown in soil in the same container, withheld from water for 15 d and then re-watered. The photographs were taken 36 h after re-watering.

(B) Expression of ABA and stress-responsive genes in WT and pub19-1 mutant seedlings treated by drought stress for 3 h. Total RNA was extracted from seedlings after different treatment times. Total RNA (10 μg) was loaded in each lane and analyzed by RNA gel blots hybridized with gene-specific probes.

(C) Ten fresh leaves from WT or pub19-1 mutant plants were incubated under light in buffer for 3 h and then treated with 0 and 5 μM ABA for 5 h before aperture measurements. Data are mean ratios of width to length ± SE of three independent experiments (n = 30–40).

(D) Stomatal apertures of WT and pub19-1 mutant plants. Stomatal guard cells were observed in the epidermal peels treated with 0 or 5 μM ABA. Bar = 5 μm.