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. 2011 Apr 18;4(6):938–946. doi: 10.1093/mp/ssr030

Figure 7.

Figure 7

Drought-Tolerance Assays of Control and Overexpressing Plants and Effects of ABA on Stomatal Aperture of Vector Control and PUB19-OX1 Plants.

(A) Two-week-old plants were used for drought-tolerance assays. Plants were grown in soil in the same container, withheld from water for 15 d, and then re-watered. The photographs were taken 5 d after re-watering. Vector: control plants, OX-1 and OX-2: transgenic plants.

(B) Expression of ABA and stress-responsive genes in vector control and PUB19-OX1 seedlings treated by drought stress at different times. Total RNA was extracted from seedlings for 1.5 h. Total RNA (10 μg) was loaded in each lane and analyzed by RNA gel blots hybridized to gene-specific probes.

(C) Ten fresh leaves from vector control or PUB19-OX1 plants were incubated under light in buffer for 3 h and then treated with 0 and 5 μM ABA for 5 h before aperture measurements. Data are mean ratios of width to length ± SE of three independent experiments (n = 30–40).

(D) Stomatal apertures of vector control and PUB19-OX1 plants. Stomatal guard cells were observed in the epidermal peels treated with 0 or 5 μM ABA. Bar = 5 μm.