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. 2011 Jan 4;26(6):983–990. doi: 10.1159/000324011

Fig. 2.

Fig. 2

Ussing chamber analysis of CFTR Cl- transport in mixed monolayer cultures of CFBE41o-/16HBE41o-cells. Original short-circuit current traces (A-E) of transepithelial Cl- currents (ICl) in (A) pure cultures of CFBE410- monolayers (100% CFBE41o-/0% 16HBE14o-), mixed cultures of CFBE41o-/16HBE14o- cells with increasing numbers (B) 0.1%, (C) 1%, and (D) 10%, of GFP-labeled 16HBE14o- cells, and (E) pure cultures of 16HBE140- monolayers (100% 16HBE14o-/0% CFBE41o-). CFTR Cl currents were step-wise stimulated by sequential additions of the CFTR Cl channel openers forskolin, 1-ethyl-benzimidazolinone (EBIO), and genistein. The CFTR blocker CFTR(inh)-172 was used to quantify CFTR-mediated ICl. Even though different y-axis scales are used (A-C vs D and E), a small (∼0.4 μA/cm2) inhibitory effect of CFTR(inh)-172 was detected, suggesting residual ΔF508-CFTR activity in 100% CFBE41o- cultures. Corresponding live cell GFP fluorescent images (F-J) of pure and mixed cell populations provide visual evidence of the relative proportion of GFP-labeled, wtCFTR expressing 16HBE14o- cells within each population; (F) 100% CFBE41o-cells, 16HBE14o-/CFBE41o-cultures containing (G) 0.1%, (H) 1%, (I) 10% GFP-labeled 16HBE14o- cells, and (J) 100% 16HBE14o- cells. (K-O) Bright-field images of corresponding monolayers. (magnification: x100).