Fig. 4.
CFTR Cl- currents in polarized monolayer mixtures of CFBE41o-/16HBE14o-. Changes in transepithelial Cl- current (ΔICl) upon: (A) stimulation with a cocktail of CFTR transport enhancers (forskolin, 2×10−5 M; EBIO, 10−3M; genistein, 10−5 M), and (B) inhibition with the CFTR blocker CFTR (inh)-172 (2×10−5 M, mucosal). The portion of normal, 16HBE14o-, cells within the population is indicated. Stimulated and blocked CFTR Cl- currents were significantly increased in mixed monolayers containing 1%, 10%, and 100% 16HBE14o- cells, respectively. (C) Graphic representation of stimulated and CFTR(inh)-172-sensitive ICl of CF cultures with varying proportions of non-CF bronchial epithelial cells. Data are presented as a percentage of the changes in ICl measured in cultures containing 100% 16HBE140- cells. Note that mixed CF monolayers with ∼10% normal cells generated ∼35-40% of the ICl observed for monolayers containing 100% normal CFTR cells. Data are expressed as mean values ± SEM; n = 2-5 experiments per group.