Fig. 1.

DTT pretreatment potentiates acid-induced currents. A. Representative traces of currents evoked by extracellular [H⁺] (pH 6.0) in VSMCs before DTT pretreatment (control), and after 5-6 min of 0.5 and 2 mM DTT pretreatment. Group data of the effect of DTT (0.5 or 2 mM) pretreatment on the normalized peak amplitude (B) and accumulated currents (C, area under curve, AUC) induced by extracellular [H⁺], pH 6.0 (n=8 and 7, respectively). Reproducibility of the potentiation effect of DTT (2 mM) on the same cell after wash-off (10-15 min). Peak current (D) and AUC (E) are not different between first and second DTT exposure (n=5). Effect of DTT (2 mM) on pH dose response for peak current (F), AUC (G), and percent responding VSMCs (H) (n=4-8). ^∗Significantly different, p<0.05.