Fig. 4.

Surgical limb appearance (a, b) and use (c, d) scores were collected at multiple time points after FSAVPR in WT (n = 12), Cx37–/– (n = 9) and Cx40–/– (n = 14) animals. Median appearance (a) and use (c) scores are shown as a function of time following FSAVPR. Asterisks indicate a significant difference in scores relative to WT at the specified time point, and premature sacrifice of necrotic animals is denoted by the new sample sizes indicated in gray. For WT (n = 11 total, n = 8 surviving to day 14), Cx37–/– (n = 7) and Cx40–/– (n = 14, n = 6 surviving to day 14) animals, appearance (b) and use (d) scores for each animal at day 14 are shown; frequency of scores are denoted in gray. In Cx37–/– animals, both median hindlimb appearance and use were only mildly affected by FSAVPR at day 1 and recovered rapidly by day 3, whereas median limb appearance and use scores for Cx40–/– mice were profoundly more negative relative to WT at most time points, indicating rapid necrosis and autoamputation in these animals.