Fig. 7.

NPP1 is more highly expressed by cementoblasts during tooth development and increased in Ank KO cementoblasts. A, B In 45 dpc WT molar, NPP1 staining was observed most strongly in cementoblasts, but was present at low levels in osteoblasts, odontoblasts and some PDL cells. C, D In Ank KO molars, NPP1 was remarkably increased in cementoblasts lining the root, as well as some cells embedded in the thick cementum, though expression was not different in other cells of the dentoalveolar complex. Scale bars represent 400 μm in A and C, and 100 μm in B and D. d = Dentin; cc = cervical cementum; p = PDL; b = bone.