Analysis of Pchlide levels in au
and yg-2. A, Room-temperature absorption and
fluorescence (inset, excitation at 440 nm) spectra of hexane-washed
acetone extracts from 5-d-old, dark-grown wild-type, au,
and yg-2 seedlings. B and C, Quantitation of
Pchlide from multiple absorption spectra in different alleles of
au and yg-2 expressed per seedling (B) or
on a fresh weight basis (C). The wild-type (WT) value represents the
mean ± se of four different backgrounds (Ailsa Craig,
Moneymaker, breeding line GT, and VF145 [Table I];
n ≥ 3 for each). All other values are means ±
se (n ≥ 3).