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. 1999 Jan;119(1):143–152. doi: 10.1104/pp.119.1.143

Figure 5.

Figure 5

Analysis of porphyrin synthesis in au and yg-2 mutants following incubation in porphyrin precursors. A, Room-temperature absorption and fluorescence (inset, excitation at 440 nm) spectra of hexane-washed acetone extracts from dark-grown wild-type (WT), au, and yg-2 seedlings incubated in the dark for 20 h in 10 mm ALA. B, Quantitation of Pchlide and Proto IX following incubation in 10 mm ALA or 10 mm glutamate (glu). Values are means ± se (n ≥ 3). The data for no addition (no add.) are from intact seedlings and are the same as those shown in Figure 3 (au and yg-2 only).