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Analysis of Mg-protoporphyrin synthesis in au and yg-2 mutants following incubation in the iron chelator 2′2′-bipyridyl (BP). A, Room-temperature fluorescence spectra (excitation at 410 nm) of hexane-washed acetone extracts from dark-grown wild-type (WT), au, and yg-2 seedlings incubated in the dark for 20 h in 10 mm 2′2′-bipyridyl. B, Quantitation of Mg-protoporphyrin from multiple fluorescence spectra. Values are means ± se (n = 3).
