Fig. 2.

Flow diagram of recruitment and performance analysis process. Bocavirus that were not detectable in both clinical virology laboratory and secondary RT-PCR based platform were excluded (2 subjects with bocavirus only detection and 2 bocavirus detection). After excluding bocavirus and coronavirus detections that clinical virology laboratory had no protocol to detect, 188 were included in the primary performance analysis as in Table 3. Six coronavirus and 31 samples for which clinical virology laboratory and RT-PCR/ESI-MS did not agree with sufficient volume left were sent to secondary RT-PCR based assay for secondary analysis.