Table 2.

Performance of the RT-PCR/ESI-MS platform for individual pathogens compared to clinical virology methods only (A) and conventional virology with secondary RT-PCR based methods combined (B).

Pathogens	A			N = 188		B	N = 194
	TP	FP	FN	Raw sensitivity (%)	Raw specificity (%)	2nd TP	2nd analysis sensitivity (%)	2nd analysis specificity (%)
RSV	9	10	2	81.8	94.4	15	88.2	97.7
Parainfluenza	4	0	0	100	100	4	100	100
Influenza	26	15	2	92.9	90.6	36	94.9	96.8
Adenovirus	2	4	1a	67	97.8	3	75	98.4
Coronavirusb	NA	NA	NA	NA	NA	4	100	98.4

TP: true positive, RT-PCR/ESI-MS (+) and clinical virology (+).

FP: false positive, RT-PCR/ESI-MS (+) and clinical virology (−).

FN: false negative, RT-PCR/ESI-MS (−) and clinical virology (+).

2nd TP: secondary analysis true positive, RT-PCR/ESI-MS (+) and clinical virology (+) or secondary RT-PCR based method (+).

NA: not available.

^aOne coronavirus isolation that detected by conventional virology reference test as adenovirus.

^bExcludes one coronavirus detection with insufficient volume to send to the secondary PCR method.