Figure 2.
Man-BSA inhibits JNK and p44/p42 MAPK activation induced by T. cruzi. Peritoneal cells (2x106cells/well) were cultured with Man-BSA (250, 500 and 750 ng/ml), mannan (2.5, 5 or 10 ug/ml) or medium (-) for 2 h and then infected with Tp of T. cruzi (three parasite per cell) for 30 min. Whole-cell lysates were resolved by SDS-PAGE (10%), followed by Western blot using antibodies against phospho-JNK, phospho-p38, phospho-p44/p42 or β-actin. Peritoneal cells treated with FBS (20%, 30 min) were used as control for p44/p42 phosphorylation (A), UV-treated cells were used as control for JNK phosphorylation (B) and NaCl-treated cells (300mM, 30min) were used as control for p38 MAPK phosphorylation (C)