Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 1;7(9):1345–1356. doi: 10.7150/ijbs.7.1345

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

Functional characterization of the recombinant AcNPV-LC3 baculovirus and schedule of immunization. AcNPV-LC3 baculovirus was generated by cloning the sequence of the E. histolytica Gal-lectin LC3 fragment into the pBlueCMV transfer plasmid downstream of the CMV promoter sequence (A). Ability of AcNPV-LC3 to drive the expression of LC3 in mammalian cells was carried out by Western blot on transduced HepG2 cells extract using a rabbit polyclonal anti-Gal-lectin heavy subunit antiserum. A band of the expected LC3 molecular weight of 50 kDa was observed in the transduced cells (B, line 3). No band was observed in non-transduced cells extract (B, line 2). Details on schedule immunization (oral and nasal routes), intraportal challenge with amoeba and sacrifice of hamsters is shown in C.