TABLE 1.

ExL Settings

	Conventional run	ExL run
	DE	DE	FE (uromodulin)
ExL size	50	50	20
Exclusion duration(s)	120	120	Continuous
PRM	−1.5 to 1.5	−1.5 to 1.5	−0.07 to 0.09a

^aFor FE of uromodulin peptides, the continuous use of a PRM exclusion window similar to that used in DE resulted in an unacceptably low number of protein identifications (data not shown). This was circumvented by narrowing the exclusion window markedly as shown.