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Reperfuslon following 24 or 48 hours of cold preservation resulted In a significant burst of superoxide generation (C and F). Levels of superoxide were significantly greater (P < .001, P < .0001) than at the end of cold ischemia alone. Significantly higher superoxide levels were observed on reperfusion following 48 hours (F) of preservation, than the levels found after 24 hours (C; P < .001).
