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. 2011 Sep 17;20(24):4797–4809. doi: 10.1093/hmg/ddr418

Figure 3.

Figure 3.

The extracellular domain of IL1RAPL1 interacts with that of PTPδ. (A) Two sets of HEK293FT cells were transfected with IL1RAPL1 or IL1RAPL1▵N and with the Myc-tagged constructs of LAR-ecto-pDis (LAR), PTPδ-ecto-pDis (PTPδ) and PTPσ-ecto-pDis (PTPσ) for 16 h using the calcium phosphate precipitation method. Cells expressing the proper construct were plated together on a 16 mm cover slip and grown for 24 h before fixation and staining with IL1RAPL1 or Myc (35). (B) Cells were transfected as described in (A), and the lysate was mixed 1:1 and immunoprecipitated with antibodies against IL1RAPLA1. The eluent was analyzed by western blot using IL1RAPL1 and Myc antibodies.