Figure 7.

Dendritic spine number and morphology of hippocampal neurons are regulated by RhoGAP2. (A) Hippocampal neurons at DIV 9 were transfected with GFP alone or with GFP and Myc-RhoGAP2 or GFP and Myc-RhoGAP2▵C, as indicated on the panels. After 1 week, neurons were fixed and stained for GFP and Myc. (B–D) Quantification of the mean (±SEM) length (B), width (C) and number (per 10 μm) of dendritic spines. Over 14 transfected neurons from four independent experiments were measured for each transfection (*P < 0.01, Student's t-test) (scale bar = 10 µm).