Table 1.

Primers used for STR amplification

STR Marker	Forward Primer (5'-3')	Reverse Primer (5'-3')	Primer μM
D8S1106	[FAM]-GTTTACCCCTGCATCACTGG	GTCAGAATTGCTCATAGTGCAAGA	0.045
DYS389	[FAM]-CCAACTCTCATCTGTATTATCTATG	GTCTTATCTCCACCCACCAGA	0.200
D1S518	[VIC]-GCAGATCTTGGGACTTCTCG	GTGTGGGCAACTGCATTAGAG	0.420
D6S1017	[VIC]-CTGGCACAGGATAGGTGCTC	GATTGAACCAGATGGGAACGA	0.300
D17S1304	[NED]-ACCATGTCCTCTGGTTCTGG	GTTTCTTACAGGTGGGACTTGGTGAAA	0.040
D4S2408	[NED]-TCATTTCCATAGGGTAAGTGAAAA	GCCATGGGGATAAAATCAGA	0.200
D5S1467	[PET]-GCCTAAGGTGGTGAATTGGA	GTGCATTATTGGAGGCTTTCTC	0.060
D19S245	[PET]-GACCTGCAATCAGCCATTTT	GTTCTTGCAGTCTGTGGCTTG	0.360

Reverse primers that did not end in a guanine base at the 5'end were modified by adding an additional guanine (G) base or a "PIGtail" sequence (GTTTCTT) to the 5' end of the reverse primer (underlined) to promote complete adenylation [37]. Primer concentrations listed are final concentrations of forward and reverse primers in a 20 μL reaction volume. Primer concentrations were determined empirically based on peak height, DNA concentration, and number of cycles in the PCR program.