Table 1.
STR Marker | Forward Primer (5'-3') | Reverse Primer (5'-3') | Primer μM |
---|---|---|---|
D8S1106 | [FAM]-GTTTACCCCTGCATCACTGG | GTCAGAATTGCTCATAGTGCAAGA | 0.045 |
DYS389 | [FAM]-CCAACTCTCATCTGTATTATCTATG | GTCTTATCTCCACCCACCAGA | 0.200 |
D1S518 | [VIC]-GCAGATCTTGGGACTTCTCG | GTGTGGGCAACTGCATTAGAG | 0.420 |
D6S1017 | [VIC]-CTGGCACAGGATAGGTGCTC | GATTGAACCAGATGGGAACGA | 0.300 |
D17S1304 | [NED]-ACCATGTCCTCTGGTTCTGG | GTTTCTTACAGGTGGGACTTGGTGAAA | 0.040 |
D4S2408 | [NED]-TCATTTCCATAGGGTAAGTGAAAA | GCCATGGGGATAAAATCAGA | 0.200 |
D5S1467 | [PET]-GCCTAAGGTGGTGAATTGGA | GTGCATTATTGGAGGCTTTCTC | 0.060 |
D19S245 | [PET]-GACCTGCAATCAGCCATTTT | GTTCTTGCAGTCTGTGGCTTG | 0.360 |
Reverse primers that did not end in a guanine base at the 5'end were modified by adding an additional guanine (G) base or a "PIGtail" sequence (GTTTCTT) to the 5' end of the reverse primer (underlined) to promote complete adenylation [37]. Primer concentrations listed are final concentrations of forward and reverse primers in a 20 μL reaction volume. Primer concentrations were determined empirically based on peak height, DNA concentration, and number of cycles in the PCR program.