Table 2.

Sequencing primers used for sequence verification and repeat number determination

STR Marker	Sequencing Primers (5'-3')	Amplicon Size (bp)	Tm (°C)
D8S1106	F- GTTTACCCCTGCATCACTGG R- GGTGGCCTAACCAGAGTTGA*	267-291	55
DYS389	F - CCAACTCTCATCTGTATTATCTATG R - GTCTTATCTCCACCCACCAGA	321-337	60
D1S518	F - GCAGATCTTGGGACTTCTCG R - GTGTGGGCAACTGCATTAGAG	182-198	55
D6S1017	F - CTGGCACAGGATAGGTGCTC R - GATTGAACCAGATGGGAACGA	354-374	59
D17S1304	F - ACCATGTCCTCTGGTTCTGG R - GTTTCTTACAGGTGGGACTTGGTGAAA	197-213	60
D4S2408	F - TCATTTCCATAGGGTAAGTGAAAA R - GCCATGGGGATAAAATCAGA	336-360	60
D5S1467	F - GCCTAAGGTGGTGAATTGGA R - GAAGATGGCCCATTTCCATTT*	313-329	60
D19S245	F - GACCTGCAATCAGCCATTTT R - GTTCTTGCAGTCTGTGGCTTG	225-249	60

*New primers designed specifically for sequencing purposes. All other primers are original primers developed for the multiplex assay.