Table 2.
STR Marker | Sequencing Primers (5'-3') | Amplicon Size (bp) | Tm (°C) |
---|---|---|---|
D8S1106 | F- GTTTACCCCTGCATCACTGG R- GGTGGCCTAACCAGAGTTGA* |
267-291 | 55 |
DYS389 | F - CCAACTCTCATCTGTATTATCTATG R - GTCTTATCTCCACCCACCAGA |
321-337 | 60 |
D1S518 | F - GCAGATCTTGGGACTTCTCG R - GTGTGGGCAACTGCATTAGAG |
182-198 | 55 |
D6S1017 | F - CTGGCACAGGATAGGTGCTC R - GATTGAACCAGATGGGAACGA |
354-374 | 59 |
D17S1304 | F - ACCATGTCCTCTGGTTCTGG R - GTTTCTTACAGGTGGGACTTGGTGAAA |
197-213 | 60 |
D4S2408 | F - TCATTTCCATAGGGTAAGTGAAAA R - GCCATGGGGATAAAATCAGA |
336-360 | 60 |
D5S1467 | F - GCCTAAGGTGGTGAATTGGA R - GAAGATGGCCCATTTCCATTT* |
313-329 | 60 |
D19S245 | F - GACCTGCAATCAGCCATTTT R - GTTCTTGCAGTCTGTGGCTTG |
225-249 | 60 |
*New primers designed specifically for sequencing purposes. All other primers are original primers developed for the multiplex assay.