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. 2009 Aug;23(8):2710–2726. doi: 10.1096/fj.08-127696

Figure 5.

Figure 5

No cell death, but increased heat shock protein expression after proteasome inhibition overnight. A, B) DAPI staining did not show any increase in nuclear condensation after proteasome inhibition with 100 nM epoxomicin (epox) for 16 h (B) compared with DMSO-treated U343 cells (A). C) An MTT assay revealed no significant changes in cell viability after treatment with proteasome inhibitors epoxomicin (n=4) and lactacystin (n=3) for 16 h, compared with DMSO-treated cells. D) Hsp70 mRNA levels were significantly increased after overnight treatment with proteasome inhibitors epoxomicin (100 nM), lactacystin (20μM), MG132 (1 μM), and MG262 (125 nM) (n=3). E, F) Hsp70 IR was clearly increased after 100 nM epoxomicin treatment (F) compared with DMSO treatment (E). G) Hsp70 levels increased significantly when lactacystin concentrations exceeded 20 μM. Because GFAP transcript levels were already maximally reduced at 20 μM, the induced stress response does not seem to influence the effect on GFAP mRNA (n=1). Scale bars = 50 μm. Data represent means ± se between individual experiments. *P ≤ 0.05. Norm., normalized.